Received July 23, 2020
Accepted January 05, 2021

Purpose:Determine if the reproductive toxicity can be reduced by off-gassing the plasticware before use for in vitro fertilization culture. Methods: Petri dishes were grouped according to the off-gassing days before use for testing: 0 day (Group A), 3 days (Group B), 7 days (Group C). Two bioassays were tested: Human survival sperm assay (HSSA) at 24 hours was carried out for all groups and mouse embryo assay (MEA) was also used to evaluate the effect of off-gassing procedure. Sperm motility and sperm motility index values were examined, blastocyst formation was calculated on day 4 and 5 as well. Results: The HSSA showed the motility of the sperms of the group A (84%) were reduced compared to groups B (94%) and C (0.93%) (p< 0.5). The MEA showed no statistical differences of the blastocyst formation for the off-gassing groups (79.6%, 84.4%, 80%, Group A, B and C respectively; p> 0.5). Hatching blastocyst formation on day 5 was reduced in non-off-gassing group (49% vs 56.7%, p>0.5). Conclusion: The benefit effect from off-gassing at least 72 hours reduce the toxicity of the plasticware before use for in vitro fertilization culture for HSSA. Further investigation needs to be done in order to standardize the bioassays used to evaluate this procedure.