Zeinab Rafiee, Fatemeh Rezaee-Tazangi, Leila Zeidooni, Hadis Alidadi, Layasadat Khorsandi
JBRA Assist. Reprod. 2021; 25 (3):459-465
Received August 08, 2020
Accepted February 23, 2021
Abstract
ABSTRACT
Objectives: This study aimed to explore the impacts of selenium (SE) on Bisphenol-A (BPA)-exposed sperm and testicular isolated mitochondria of mice.
Methods: Mouse sperms and isolated mitochondria exposed to BPA (0.8 mM) and different concentrations of SE (50, 100, and 200 µM) for 4 hours. The viability of sperms and isolated mitochondria as well as mitochondrial membrane potential (MMP) was evaluated. SOD (superoxide dismutase), GSH (glutathione), MDA (malondialdehyde), and ROS (reactive oxygen species) levels of the testicular mitochondria were also examined.
Results: BPA concentration-dependently enhanced ROS level and MDA amount in the isolated mitochondria while MMP and acclivities of the GSH and SOD significantly reduced. BPA also impaired considerably survival rate and motility of the spermatozoa. SE could concentration-dependently reverse mitochondrial oxidative stress, MMP, sperm survival, and total sperm motility.
Conclusion: our findings collectively suggest that SE concentration-dependently reverses the BPA-caused mitochondrial toxicity and reduced sperm motility by suppressing oxidative stress.
