Ria Margiana, Silvia Werdhy Lestari, Kamila Alawiyah, Pety Narulita, Ahmad Aulia Jusuf, Khoirul Ima
JBRA Assist. Reprod. 2023; 27 (2):147-155
Received September 25, 2021
Accepted April 01, 2022
Abstract
This study aimed to determine the effect of soybean administration on the expression of ZP2 in female mice. This research used Mus musculus, six-week-old female SWISS strain mice divided into three groups (group without soybean administration and groups with mixed feed with soybeans and pelleted 50:50 and 25:75). Soybean feed for mice was 360 grams per kilogram of body weight of the mice for 2 weeks. The percentage of follicles was measured and analyzed using Hematoxylin-Eosin staining, and the expression of ZP2 was analyzed using immunohistochemistry. Data were evaluated using one-way ANOVA and paired t-test on SPSS 17. Some of the follicles in the ovaries do not develop until the final stage of follicle maturation. The administration of soybean before and after treatment in all groups was insignificantly different, but the numbers of atretic follicles in groups 1 and 2 were significantly different. Soybean administration with a ratio of 50:50 has the effect of increasing the percentage of the ZP2 expression in tertiary follicles (p = 0.001), whereas soybean administration with ratio of 25:75 has not been able to maintain or increase the formation of ZP2 in tertiary follicles (p = 0.77).
