Received January 08, 2022
Accepted July 20, 2022

Objective: The present study was conducted to consider the effect of aqueous and hydro-alcoholic extracts of Seidlitzia rosmarinus on reproductive hormones, sperm variables, and antioxidant enzymes level in the mice testis. Methods: In this experimental study, 24 three-month-old male NMRI mice weighing (25-30g) were divided into three groups: control, aqueous and hydro-alcoholic extracts of Seidlitzia rosmarinus 100 mg/kg. Dissolved extracts were gavaged orally for 35 days. One day after receiving the last dose of the extract, the blood sample, testis, and the caudal of epididymis were taken for plasma hormonal, testicular antioxidants level, sperm count, and vitality assessment. Results: Testicular level of malondialdehyde increased in aqueous and hydro-alcoholic extracts groups (p=0.04), total antioxidant capacity decreased in aqueous and hydro-alcoholic extracts groups (p=0.008), and consumption of aqueous (p<0.001) and hydro-alcoholic (p=0.03) extracts decreased catalase in comparison with the control group. The plasma level of luteinizing hormone decreased in the aqueous extracts administrated group (p=0.009), follicle-stimulating hormone increased in aqueous (p=0.03), and hydro-alcoholic extracts administered mice, and testosterone level decreased in aqueous extract-treated animals versus the control group (p<0.001). The sperm count was increased in aqueous (p=0.04) and hydro-alcoholic (p=0.009) extracts groups, but its vitality was decreased (p=0.008) in comparison with the control group. Conclusion: In conclusion, Seidlitzia rosmarinus has an adverse effect on male reproductive hormones and sperm viability via increase lipid peroxidation and reduce antioxidant defense system performance.