Rodopiano de Souza Florêncio, Pablo Rassi Florencio, Manoel João Batista Castello Girão, Edmund Chada Baracat
JBRA Assist. Reprod. 2012; 16 (6):337-342
Received December 10, 2012
Accepted December 31, 2012
Abstract
Objective: to investigate the efficacy of bovine pericardium to prevent pelvic adhesion in dogs.
Methods: Seventeen female non pregnant dogs were anesthetized and submitted to two adhesion-inducing lesions – the first lesion was performed in the anterior portion of uterus (elliptical incision) and sutured with an ischemic line of chromic catgut and the second, when closing the peritoneum, also with an ischemic suture. BP was used as the barrier agent in nine dogs, fully covering the first lesion and applied on the inferior part of the second lesion. Six days later, the dogs were sacrificed and the adhesions were examined according to the ¨More Comprehensive Adhesion Score Method¨ (MCASM, Adhesion Study Group-EUA) and to the score developed by the author in which only the percentage of the adhesion is examined, ¨Adhesion Extension in Experimental Lesion¨ (AEEL).
Results: There were no significant differences between the two groups regarding weight and surgical time in minutes . no significant differences were observed between the MCASM scores of the two groups (6.50 vs. 6.00, p= 0.718) and the AEEL (4.87 vs. 4.33, p=0.615) for lesion 1. There were significant differences in the second lesion in the two groups, regarding the mean scores given by the MCASM system (4.62 vs.7.55, p=0.005) and the adhesion extension score (2.75 vs. 4.77, p=0.015).
Conclusions: BP did not prevent the formation of adhesions caused by the lesions performed. Furthermore, BP enhanced the formation of adhesions in lesion 2.