Received August 09, 2022
Accepted March 31, 2023

The aim of this study was evaluate the mouse embryo development in vitro. Swiss female mouse(6-8 weeksage) were superovulated(5lU of eCG and 5lU of hCG) and then matted. Eighteen hours late they were sacrificed for embryo retrieval. The embryos were washed in phosphate buffer sodium (PBS) with 10% of bovine calf serum (BCS). They were co-cultivated in 6 different kinds of somatic cell primary culture was neuronium and hepatocyt(rat fetus) and bovine oviduct epithelial cell(BOEC); established cell were MDBK(bovine kidney), VERO(green mouse kidney) and RK-13 (rabbit kidney). The cell culture was kept at 5%CO2, 37°C and 90humidity and evaluations were performed after 24, 48 and 72hours.The culture media was tissue culture media 199 (TCM199) supplemented with 10% BCS and 0,22%sodium piruvate. ln part of the experiment it was added at the culture media epidermal growth factor(EGF).The data were analyzed according to number of embryos, which were able to cross the 2-cell blockage after 72h. The control group was not able to cross the blockage.The experimental group was separated according to co-culture type and presence or absence of EGF.The data showed that BOEC is the best co-culture system without EGF, with 33% of the embryos crossing the blockage. About the others co-culture system the best one was with rat hepatocyt, showing 22% of the embryos crossing the blockage without EGF and 15% with it. The co-culture system with established cell is justified by the fact that these cell promote a better sanitary support for embryos.