Received July 08, 2023
Accepted January 27, 2024

Purpose. Clinacanthus nutans (C. nutans) is a medicinal herb that most diabetes people have historically taken. It is the first study to suggest using C. nutans to enhance the quality of sperm in male mice given a streptozotocin (STZ) injection. Methods. Sixty mice were divided into two groups at the age of four weeks: group one was fed a regular diet (n=10), while group two was administered a high-fat diet (n=50) for eight weeks to develop obesity. Mice were given 100 mg/kg of STZ to produce hyperglycemia. Then, 40 hyperglycemia mice were separated into two groups: STZ (n=10) and sample (n=30). The treatment groups were administered methanolic extract of C. nutans leaves by gavage at doses of 150, 300, and 500 mg/kg of body weight for 4 weeks. Results. In contrast to the STZ group, there was a drop-in serum blood glucose and total sperm abnormalities in mice at varying doses. Catalase, glutathione s-transferase, and total anti-oxidant capacity significantly increased in the STZ mice group at varying doses, but malondialdehyde was reduced. In comparison to STZ mice, testosterone and luteinizing hormone (LH) levels improved in mice treated with extract of C. nutans at various doses. For all of the following dependent variables, extraction of the leaf at higher concentrations of 500 milligrams/kilogram has better efficacy than 300 and 150 mg/kg after 4 weeks of treatment. Conclusion. As a result, the potential leaf extract of C. nutans contains anti-hyperglycemic compounds and improves the quality of sperm in male mice.