Received September 20, 2023
Accepted December 07, 2023

Objective: Previous studies have shown that the meiotic spindle is not always aligned with the first polar body (PB) in metaphase II human oocytes. Polarized Light Microscopy (PLM) has been used to observe and locate the meiotic spindle to avoid disrupting it while injecting oocytes. The aim of this study is to evaluate the relationship between meiotic spindle view and IVF-ICSI outcomes in poor responder women. Methods: This study was a retrospective analytical study that conducted from January until November 2019 involving 115 poor responder women who underwent IVF-ICSI cycles at Halim Fertility Center, Indonesia. Patients were divided into two groups : group I without meiotic spindle viewing (non-MSV) as control group and group II with meiotic spindle viewing (MSV) as case group. Meiotic spindles were imaged before ICSI with Oosight microscopy. Baseline characteristics and IVF-ICSI outcomes of both groups were compared. Results: Our study included 115 poor responder women with non-MSV group (71 women) and MSV group (44 women). The results showed that there was no significant difference in fertilization rate between the two groups (p>0.05), but the embryo cleavage rate was higher in MSV group than non-MSV group and there was a significant difference between the two groups (p<0.05). The fertilization rate was higher in aligned than misaligned spindle and there was a significant difference between the two groups (p<0.05). Good quality embryo was higher in MSV group than non-MSV group (59.05% vs 63.95%). Conclusions: Meiotic spindle view might improve the outcome of IVF in poor responder women.