Received October 27, 2023
Accepted April 30, 2024

INTRODUCTION: Bisphenol F (BPF) is an endocrine-disrupting chemical, but information about its effect on thyroid hormones has not been fully explored. Omega 3 fatty acid (O3FA), on the other hand, is an antioxidant and antiapoptotic agent. Therefore, this study explored the role and associated molecular mechanism of O3FA in BPF-induced hypothyroidism-mediated testicular dysfunction in male Wistar rats. METHODS: Twenty (20) male Wistar rats were randomized into four (4) groups (n=5/group); the control group, BPF treated group received 30 mg/kg of BPF, and the intervention groups received 30 mg/kg BPF + 100 mg/kg O3FA (BPF+O3FA-L) and 30 mg/kg BPF + 300 mg/kg of O3FA (BPF+O3FA-H) for 28 days. RESULTS: Low and high doses of O3FA ameliorated BPF-induced hypothyroidism mediated reduction in sperm quality, testosterone, luteinizing hormone, follicle-stimulating hormone, catalase, superoxide dismutase, total antioxidant capacity, and nuclear factor erythroid 2–related factor 2 and increase in estrogen, malondialdehyde, c-reactive protein, interleukin 1 beta, caspase 3. Furthermore, O3FA prevented BPF-induced hypothyroidism Na+/K+-ATPase and Ca2+-ATPase dysfunction, estrogen receptor beta overexpression, and tumor protein P53 (p53)/ b-cell lymphoma 2 (Bcl-2) imbalance. CONCLUSION: This study showed that O3FA ameliorated BPF-induced dysthyroidism-mediated testicular dysfunction by preventing proton pumps dysfunction and p53/BCl-2 imbalance