Gabriel de Moura, Mayara Lobato Lourenço, Yasmim Mendes Rocha, João Pedro Viana Rodrigues, Cristian Vicson Pinheiro, Alice Soares de Queiroz, Eduardo de Paula Miranda, Sebastião Evangelista Torquato Filho, Roberto Nicolete
JBRA Assist. Reprod. 2024; 28 (3):457-463
Received October 10, 2023
Accepted April 30, 2024
Abstract
Background: One of the techniques that has gained much attention is the in vitro maturation of oocytes for patients who use assisted reproduction techniques. However, its results are still inferior to controlled ovarian stimulation methodologies. Understanding the maturation mechanisms based on analyses can help improve this methodology's results. Objective: The work aims to identify the central genes differentially expressed in oocytes after in vitro maturation in the germinal vesicle and metaphase II stages. Results: Two datasets were identified on the Gene Expression Omnibus database platform: registration data GSE158802 and GSE95477. From the analysis, we identified five downregulated and thirty-six upregulated genes; the central genes that correlated with the main gene proteins found were CLTA and PANK1. Conclusion: There was a differential regulation of gene expression. The most central ones are related to energy capture.
