Received May 13, 2024
Accepted November 30, 2024

Objectives: The study focused the contraceptive efficacy of hydro-ethanolic (60:40) extracts (HEE) of Ceasalpinia pulcherrima leaves in human and rat sperm samples by in-vitro study. Materials and methods: Six young fertile adult males were selected for semen collection. Sperm samples were collected from six adult rats also by chopping the epididymis along with the collection of testicles, other epididymis, and liver. The semen, sperm, and tissue samples were grouped into control, 1, 2, and 4 mg HEE exposed categories. Sensitive spermiological sensors, androgenic key enzymes, oxidative stress, and metabolic toxicity markers were assessed according to standard protocols. Human semen samples, rat�s sperm samples and metabolic tissue samples were divided into 16 test tubes in all of the above groups to find out the direct effect of the extract on such sensors in concentration and duration dependent manner. Results: Spermiological sensors both in human and rat were decreased significantly (p<0.05) in concentration and duration dependent manner after in-vitro exposure of HEE against the control group. Testicular Δ5, 3β-hydroxysteroid dehydrogenase, 17β- hydroxysteroid dehydrogenase, superoxide dismutase, and catalase activities were significantly (p<0.05) decreased, and level of the end product of lipid oxidation-peroxidation was elevated (p<0.05) in rat after extract charging. No general toxicity imposition of the said extract indicated by the activities of hepatic transaminases. Phytochemical screening was done by qualitative and liquid chromatography-mass spectrometry analysis. Conclusion: Extract focused the promising male contraceptive potentiality at 2 mg/ml concentration. Mode of action will be unfolded from running in-vivo study very shortly.