Subfertility affects up to 18% of couples, and poor semen quality has been implicated in approximately half of the cases. Semen analysis has been universally used to assess semen quality. In 2010, the World Health Organization (WHO) defined new reference values for sperm parameters to discriminate between normal and abnormal semen samples (including oligozoospermia, asthenozoospermia, teratozoospermia, and the combinations of these factors) (Cooper et al., 2010). However, reports suggest that the prognostic relevance of the 2010 WHO classification system is poor (Esteves et al., 2012; Hamilton et al., 2015). Semen parameters such as volume, concentration, and motility can be pooled to yield a different manner to express sperm quality: the total motile sperm count (TMSC). The TMSC is obtained by multiplying the volume of the ejaculate by the sperm concentration by the proportion of progressive motile sperm divided by 100% (Ayala et al., 1996), and can be calculated from the neat semen (prewash TMSC) or after semen preparation (postwash TMSC).