Received April 14, 2025
Accepted November 25, 2025

Objective: To examine the relationship between oxidative stress markers and reproductive parameters in women undergoing assisted reproductive treatments, focusing on the interaction between demographic, oxidative, and reproductive factors. Methods: A total of 49 patients with a mean age of 36.0±3.5 years were evaluated. Biomarkers analyzed included reduced glutathione (GSH), disulfide glutathione (GSSG), total glutathione (tGSH), the GSSG/GSH ratio, reactive oxygen species (ROS), and reactive nitrogen species (RNS). Blood and follicular fluid samples were collected and analyzed, correlating these markers with variables such as antral follicle count (AFC), oocytes retrieved, mature oocytes, blastocyst formation, and pregnancy rates. Results: The results showed that AFC is a key marker of ovarian reserve, correlating positively with the number of oocytes retrieved and mature oocytes. Age significantly influenced antioxidant markers in both blood and follicular fluid, with a decline in antioxidant capacity linked to aging. In follicular fluid, higher GSSG levels correlated with the number of oocytes retrieved, indicating increased metabolic activity and ROS generation, thus raising oxidative burden during ovarian stimulation. Additionally, GSH and tGSH levels in blood negatively correlated with mature oocytes, suggesting greater antioxidant consumption during elevated metabolic demand. Conclusions: Oxidative stress negatively impacts systemic and follicular environments, correlating with reduced oocyte quality and blastocyst formation. Age significantly affects oxidative stress, with older women showing lower antioxidant capacity. Despite elevated oxidative stress, some patients achieved successful pregnancies, underscoring the complexity of oxidative stress dynamics and the importance of personalized treatment strategies.