Received April 20, 2013
Accepted May 24, 2013

The human growth hormone plays a very important role in the final stages of oocyte maturation and embryogenesis, as well as for many other mammalian species. Patient 40 years, trying to become pregnant for 4 years: man with moderate oligozoospermia, five IVF cycles without success in other clinics. In 2009 the cycle recruited 14 oocytes, IVF was performed with embryo transfer, no pregnancy. Second fresh cycle fresh had chemical pregnancy. In the third the couple redid the tests, the husband was diagnosed with hepatitis C, and in this cycle 3 embryos were transferred, without success. On the fourth cycle the patient performed CGH, which accused trisomy, no transfer. In cycle 5 CGH got one euploid embryo, and transfer was carried out but there resulted only chemical pregnancy. In cycle 6 embryos were subjected to conventional PGD and panel with 5 chromosomes, resulting in a single euploid embryo that was transferred and resulted in negative β-HCG. After 5 months the patient started treatment again with a long protocol and use of human growth hormone: 15 follicles were aspirated. ICSI was performed for all viable oocytes. All embryos which developed were subjected to PGD on day 3, resulting in 4 euploid embryos that were transferred and resulted in β-HCG positive. Although several articles suggest various protocols, there is not a standard. Thus, studies should continue to standardize that new protocol that may result in increased chances of successful pregnancy.