Received October 21, 2025
Accepted June 05, 2026

Objective: To investigate the effects of quercetin supplementation in aging mice on the oocyte number and in vitro fertilization outcomes. Methods: Thirty-two to forty-week-old aging female mice were randomly assigned into two groups, with one receiving quercetin 30 mg/kg in 0.2 mL water orally via gavage for 21 consecutive days. The control group was given 0.2 mL of water without quercetin. In vitro fertilization was performed after exogenous ovarian stimulation. The outcome was oocyte number and quality, as indicated by oocyte fragmentation rate, fertilization rate, blastocyst formation rate, and blastocyst cell number. Results: There was no difference in the number of retrieved oocytes per mouse (17.4 ± 2.6 and 16.2 ± 1.7), oocyte fragmentation rate (30.8% and 26.7%), fertilization rate (43.5% and 43.1%) and blastocyst formation rate (32.6% and 33.9%) in the quercetin group compared to the control group. Likewise, the mean numbers of cells in the ICM, TE, and total cell number in blastocysts from both groups were not different. Conclusion: Dietary quercetin supplementation did not show any beneficial effect on the aging ovary in quantity and quality. Therefore, it does not enhance IVF outcomes on aged oocytes.