Received November 15, 2013
Accepted November 15, 2013

OBJECTIVE: The human growth hormone plays a very important role in the final stages of oocyte maturation and embryogenesis, as well as for many other mammalian species. METHODS: Case report. Patient 40 years, trying pregnancy for 4 years: moderate oligozoospermia, five previous IVF cycles without success in other clinics. In 2009 recruited 14 oocytes, IVF was performed with embryo transfer, not pregnant. Second fresh cycle had only chemical pregnancy. In the third the couple redid the tests, the husband was diagnosed with hepatitis C, and on this cycle 3 embryos were transferred, without success. On the fourth cycle patient performed CGH, which accused trisomy, no transfer. In cycle 5 CGH was performed and resulted one euploid embryo, and again only chemical pregnancy. We performed cycle 6 and embryos that developed were subjected to conventional PGD and panel with 5 chromosomes, resulting in a single euploid embryo that was transferred and resulted in negative β-HCG. After 5 months the patient started treatment again with long agonist protocol and use of human growth hormone. RESULTS: 15 follicles were aspirated. ICSI was performed for all viable oocytes. All embryos which developed were subjected to PGD on day 3, resulting in 4 euploid embryos that were transferred and resulted in β-HCG positive. Although there are several articles that suggest different protocols, there is standardization. Thus, studies should continue to standardize that protocol that may result in increased chances of successful pregnancy.