Received November 16, 2013
Accepted November 16, 2013

OBJECTIVE: To review the experience of eight years of application of the technique of vitrification of human oocytes in the center of reproduction pioneer in Mexico. MATERIAL AND METHOD: We included the cycles of oocyte vitrification conducted from July 2004 to December 2012 in the Mexican Institute of infertility of Guadalajara, Jalisco, Mexico. Patients or donors were stimulated with recombinant FSH (r-FSH) or urinary FSH (u-FSH) starting on day 2 or day 3 of menstrual cycle. Oocyte aspiration was performed 36 hours after the application of 10,000 IU of HCG. TECHNIQUE: After removing the cumulus from the oocytes, the oocytes were deposited in equilibrium solution for 10 minutes. They were transferred to a vitrification solution, and then placed on the cryotop. For warming, the eggs were deposited in heating and washing (Kit-Kitazato) solution. The surviving oocytes were microinjected using ICSI. RESULTS: The rate survival of 2,346 oocytes for 513 transfer cycles was 90.9%. The proportion of pregnancy per patient (29.5%) (135/458) (Table 1). In Figure 1, we observed the distribution of cycles, and number of oocytes vitirfied per year. There was a decline in egg survival when 8 or more oocytes vitrified per cryotop (Figure 2). Have borned 118 babies in eight years, 3 (2.54%) babies had malformations. CONCLUSION: Cryotop vitrification allows high rates of egg survival. Similar to those obtained in fresh pregnancy rates. Live births without increase in the proportion of congenital malformations.