Maria Guadalupe De Alba Cervantes, Diego Carrillo Ruiz Velasco, Francisco Rocha Cardenas, Rocio Martinez Armas, Luis Arturo Ruvalcaba Castellon
JBRA Assist. Reprod. 2013; 17 (2):128-128
Received November 16, 2013
Accepted November 16, 2013
Abstract
OBJECTIVE: Evaluate embryonic development, after blastomere biopsy of one or two, for Preimplantation Genetics Diagnosis.
MATERIALS AND METHODS: Retrospective study of May 2011- January 2013. We analyzed a total of 121 embryo in fresh and 104 thawing. They were divided into 2 groups: 1) Biopsy of 1 blastomere and 2) Biopsy of 2 blastomeres. For corresponding cycles in fresh, were analyzed 1) 76 and 2) 45 embryos. For thawing cycles were 1) 61 and 2) 43 embryos. The results were evaluated with T-student and was considered statistically significant at p <0.05, with a confidence interval of 95%.
RESULTS: The percentage of arrested embryos per group was 1) 53.9% and 2) 48.8% in fresh cycles. For embryos thawing was 1) 80% and 2) 41%. The percentage of embryos abnormal in fresh was 1) 43% and 2) 44%, and for embryos thawing, the number of embryos abnormal was 1) 60% and 2) 62% respectively.
CONCLUSIONS: Our results showed that the embryos corresponding to cycles in fresh and thawing in to the Group 1, had a higher incidence of arrested embryos, this may be attributable to that there was a greater quantity of embryo grade 4. For cycles thawing , we found more abnormalities, in both groups 1 and 2, letting us see that vitrification could have implications in embryonic development from the biopsy of one or two blastomeres.
