Claudia G Petersen, Ana L Mauri, Laura D Vagnini, Adriana Renzi, Bruna Peteren, Marina C Mattila, Vanessa A Comar, Juliana Ricci, Felipe Dieamant, Joao Batista A Oliveira, Ricardo Baruffi, José Gonçalves Franco Jr
JBRA Assist. Reprod. 2019; 23 (1):33-36
Received March 19, 2018
Accepted October 10, 2018
Abstract
Objective:A variety of studies randomizing women/cycles or oocytes/embryos have been conducted to compare different culture media for culturing embryos up to cleavage or blastocyst stages showing controversial results. A recent systematic review suggested that data in literature are insufficient to conclude the best culture medium for embryo quality, pregnancy and implantation. The objective of this study was to evaluate if there is any difference between two commercial culture medium regarding clinical outcomes after IMSI cycles.
Material and Methods: A total of 120 patients, ≤39 years, undergoing ART treatment submitted to IMSI program were divided prospectively and at random into two groups: Group I (Cook media) and Group II (Vitrolife media).
Results: Our data demonstrated that there is no difference using all the medium from Cook or all the medium from Vitrolife, for culturing embryos till day 2 in the bench incubator at low O2 concentration, in relation of fertilization, embryos quality, pregnancy and implantation rates.
Conclusion: Both culture media used, Cook medium and Vitrolife medium, for the IMSI procedure and for later embryo culture with transfer on the second day are equally effective and can be used depending on the ease and availability of acquisition.
