Received February 02, 2018
Accepted August 31, 2018

Objective: It has been shown that in vitro maturation can influence gene expression in oocytes but the common drawback of such reports is the use of different donors for each experimental group without considering the donor effect. This study aimed to investigate the abundance of mRNA in oocytes matured in vivo or in vitro obtained from the same group of donors. Methods: Bovine model was used to evaluate the relative abundance of specific transcripts in oocytes collected from the same donors by transvaginal ovum pick-up (OPU) and matured in vitro (IN VITRO-OPU) or in vivo (IN VIVO-OPU). Transcripts abundance between oocytes from IN VIVO-OPU group and oocytes matured in vitro but recovered from different cows slaughtered at commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and reverse transcribed to cDNA using oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. Results: Oocytes of IN VITRO-OPU group had lower (P<0.05) abundance of Peroxiredoxin 1 (Prdx1), Heat shock protein 70.1 (Hsp70.1), Growth and differentiation factor 9 (Gdf9) and Maternal antigen that embryo requires (Mater) transcripts than those of IN VIVO-OPU, both obtained from the same pool of donors cows. Similar result was found when the comparison was performed between IN VIVO-OPU and IN VITRO–Abattoir groups (P<0.05). Conclusion: In vitro maturation affects the abundance of polyadenylated transcripts in the oocyte cytoplasm when compared to in vivo maturation induced by means of exogenous hormones, even whether the oocytes are from the same donors.