Toluwase Solomon Olawuyi, Kolade Busuyi Akinola, Sunday Aderemi Adelakun, Babatunde Ogunlade, Grace Temitope Akingbade
JBRA Assist. Reprod. 2019; 23 (2):117-122
Received July 07, 2018
Accepted February 01, 2019
Abstract
Objectives: The aim of this study was to investigate the effect of Lawsonia inermis aqueous leaf-extract on aluminium-induced Oxidative stress on the histology of the Pituitary gland of an adult Wistar rats’ with the objectives on histology of this Organ.
Methods: Thirty five adult male Wistar rats, weighing between 100-196g were used. Lawsonia inermis extracts and aluminum chloride (AlCl3) were administered for a period of three (3) weeks with Five (5) rats per group. Group 1 (control): Given rat pellets and distilled water. Group 2: Given 60mg/kg/d extract of aqueous Lawsonia inermis. Group 3: Given 0.5mg/kg/d of AlCl3. Group 4: Given 0.5mg/kg/d of AlCl3 and 60mg/kg/d of aqueous Lawsonia inermis orally. Group 5: Given 0.5mg/kg/d of AlCl3 and 75mg/kg/d of aqueous Lawsonia inermis orally. Group 6: Given 0.5mg/kg/d of AlCl3 and 100mg/kg/d of aqueous Lawsonia inermis orally. Group 7: Given 0.5mg/k/d of AlCl3 and 5mg/Kg/d Ascorbic acid in distilled water orally. Twenty four hours after the last administration, the animals were weighed, sedated with chloroform and Pituitary glands were located, removed and weighed using an electronic sensitive analytical balance.
Results: Pituitary gland micrograph of wistar rats given 0.5mg of aluminum chloride shown decrease in cellularities but micrograph of wistar rats given 0.5mg of aluminum chloride and varying doses of Lawsonia inermis, shown increase in cellularities which was dose dependent.
Conclusion: Lawsonia Inermis aqeuous leaf-extract increase the cellularities of the pituitary gland of adult male wistar rat. Also, alleviate oxidative stress induced by aluminum poison.
