Received November 16, 2013
Accepted November 16, 2013

OBJECTIVE: The series here reported pretends to verify in our laboratory the benefits of autologous endometrial co-culture. This technique of co-culture has been used since 2010 in 28 patients with at least one previous cycle with implantation failure (mean 1.55 +/- 0.79). METHODS: To each woman an endometrial biopsy was schedule on day 7±2 after documented the follicular rupture by transvaginal ultrasound in a spontaneous cycle using a Pipelle de Cornier (Laboratoire CCD, France). In the laboratory, endometrial stromal and glandular cells were extracted and cultured (supplemented with the patient’s blood plasma). Finally, cell suspension was criopreserved, to be used after having bacteriological results, in case they were negative. In the next ART cycle, 25 hours post insemination, the embryos were incubated over the mono layer of endometrial cells previously thawed. RESULTS: Significant differences were not found in the mean of aspirated follicles and number of oocyte retrieved, neither in the fertilisation rate nor in the quality of transferred embryos. Pregnancy and implantation rates of these patients increased significantly to 50.0% and 30.0%, respectively. CONCLUSIONS: Results demonstrate that autologous endometrial co-culture use in patients with implantation failures is beneficial on selected cases.