Received July 27, 2018
Accepted December 13, 2018

Objective: The aim of our study was to identify the prevalence of HPV in the semen of men submitted to ART treatment and to identify the possible impact of the virus in spermatic parameters. Methods: Thirty-five patients in treatment for infertility were invited to participate in the study from March to August, 2016. Samples with a minimum concentration of 40x106 spermatozoa per milliliter were included in the study. After the evaluation of the seminal parameters (WHO 2010), DNA extraction and PCR were performed to verify the presence of HPV by electrophoresis in 8% polyacrylamide gel. Results: Patients' ages ranged from 27 to 68 years (mean 39.2 years). Semen analysis showed a mean volume of 2.5 mL; mean concentration 58.9 x 106 and mean motility of 51.8%. HPV virus DNA was identified in seven semen samples from 25 patients (28%). Ten samples that were below the concentration of 10ng / μL of DNA were excluded from the study due to a loss in amplification quality. There was no statistically difference in seminal concentration between HPV negative and positive samples (65.9x106 vs 62.3x106; p = 0.70) however, sperm motility was significantly higher in the semen of HPV positive (65 vs 46.6%; p = 0.02). Conclusions: HPV prevalence was 28% in semen of patients submitted to ART. These HPV positive samples also presenting a statistically increased motility compared to negative ones (65 vs 46.6%; p = 0.02).