Cristian A Sedo, Felicitas Noblia, Florencia Nodar, Susana Kopelman, Sergio Papier, Gabriel Fiszbajn
JBRA Assist. Reprod. 2013; 17 (2):134-134
Received November 17, 2013
Accepted November 17, 2013
Abstract
OBJECTIVE: To report and identify some factors involved in fertilizations failures after ICSI.
METHODS: At the present study, 2300 human oocytes with fertilization failure after ICSI were considered. nine hundred corresponded to sub-optimal fertilization (<50%). For oocytes assessment, fixation and subsequent processing was performed. Acetylated alpha tubulin, beta tubulin and MPF (CyclinB-Cdc2) staining was performed using monoclonal antibodies. DNA detection was carried out with Hoechst-33342 and TUNEL, for fragmentation. When oocyte activation failure and sperm nuclear decondensation failure were observed, Western-Blot and immunocytochemistry were performed over semen samples for PLCzeta (oocyte activation protein) detection.
CONCLUSION: In general, fertilization failure occurs in 28% of cases for an incomplete oocyte cytoplasmic maturation. This value is increased to 50% when fertilization failures take place in ≥ 50% of the oocyte cohort. The second most recurrent event was sperm DNA decondensation failure, 39.4% depicted variable decondensation levels.
