M. Valeria Paz, Mariel Chiera, Paula Hovanyecz, Juliana Cicaré, Luciana Domenech, Viviana Ventura, Patricia Perfumo
JBRA Assist. Reprod. 2020; 24 (2):143-146
Received May 05, 2019
Accepted April 08, 2020
Abstract
Objective: To analyze the genetic and clinical outcomes of blastocysts derived from 0PN oocytes after IVF/ ICSI.
Methods: This retrospective observational study in- cluded patients aged 40 years or younger submitted to IVF/ICSI with their own oocytes and with blastocysts de- rived from 0PN oocytes between January 2015 and April 2018. The clinical outcomes of 0PN blastocyst transfers were analyzed. Genetic tests were performed on biopsied 0PN blastocysts with Next Generation Sequencing.
Results: A total of 27 0PN blastocysts were trans- ferred, yielding an implantation rate of 48.0% and an on- going pregnancy rate of 50.0%. The transfers resulted in 13 live births (59.0% live birth rate). Genetic test results revealed that four of the 17 0PN blastocysts biopsied were 46XX; three were 46XY; and 10 were aneuploid embryos, awarding a diploid rate to 76.4% (13/17).
Conclusion: Almost half of the 0PN blastocysts im- planted (48.0%) and 13 healthy babies were born. More than three quarters (76.4%) of the 0PN blastocysts were diploid, thus ruling out the possibility of parthenogenet- ic activation. Our study indicated that the transfer of 0PN blastocysts is a safe, worthy option when the number of normal 2PN embryos is insufficient.
