Jose Fernando Macedo, Maristela Rodrigues Oliveira, Luiz Mauro Oliveira Gomes, Gustavo Capinzaiki de Macedo, Giovanna Capinzaiki de Macedo, Daniela Oliveira Gomes, Camila Dutra Souza Francisquini, Bruna Oliveira Ambrogi, Sandra Irene Sprogis dos Santos
JBRA Assist. Reprod. 2020; 24 (4):411-415
Received September 20, 2019
Accepted April 01, 2020
Abstract
OBJECTIVE: Describe embryonic profile up to stage of blastocyst in a time-lapse system. DESIGN: Retrospective, longitudinal, analytical study of patients who underwent in vitro fertilization. METHODS: Embryos were grouped according to the degree of expansion, internal cell mass and trophectoderm classification, the morphokinetic parameters were related to the times observed in each evolution phase. RESULTS: The appearance of second polar corpuscle (CPap) occurred earlier in the embryos classified as excellent (2.99h; p<0.05) in relation to the embryos classified as good (3.40h), average (3.48h) and poor (3.55h). Embryos classified as excellent had less time to disappear pronuclei (PNbd) (21.80h; p<0.05) when compared to good embryos (22,96h), average (23,21h) and poor (23.47h). As for the morphokinetic parameter, the end of the division for two cells (T2) occurred first in excellent blastocysts (24.38h; p<0.05) when compared to other groups: good (25.57h), average (25.53h) and poor (25.78h). With respect to synchronization of division of three to four cells (S2), the poor embryos presented longer time for this division (3.67h; p<0.05). When compared to the embryos of excellent groups (1.97h), good (2.70h) and average (2.09h). In the time point of blastocoel formation (TB), the excellent embryos (104.04h) did not differ in relation to good embryos (104,10h). However, when compared to average (107.27h) and poor (106.86h), there was statistical significance (p<0.05). CONCLUSIONS: Embryos of better quality presented a shorter time in some morphokinetic parameters when compared to the other groups thus increasing the possibilities to establish new parameters for the classification and selection of embryos.