Received February 05, 2020
Accepted September 05, 2020

Objective: The current study was aimed at correlating of semen pH with motility and count to understand the significance of semen buffering system. Study Design: The semen samples were collected from men who visited the clinic with infertility problem. Determination of semen pH, sperm motility and count were done according to the WHO laboratory manual, 2010 standards. Mann-Whitney U-test was applied for statistical significance. The Pearson product moment correlation coefficient was used to measure the degree of linear relationship between the semen parameters. Results: For all patients (n = 310) the mean (±SD) pH was found to be 8.4 ± 0.3, with the range from 6.9 to 9.5. A significant and positive correlation was observed between total motility and pH r =0.0591 (p<0.00001); volume and pH r = 0.0582 (p<0.00001); Sluggish Progressive Motility (SPM) and pH, r = 0.0529 (p<0.00001); Abstinence and pH, r =0.0016 (p<0.00001). Negative correlation was noted between pH and total count r = - 0.025 (p<0.00001); Rapid Progressive Motility (RPM) and pH r = - 0.0776 (p<0.001), Non Motile (NM) spermatozoa and pH r = - 0.00132 (p<0.00001). Conclusion: Correlations were observed between seminal pH and two important fertility parameters, viz., motility and count indicating that semen buffering system plays vital role in maintaining the overall seminal quality, which is of clinical relevance.