Received April 24, 2020
Accepted November 03, 2020

Objective: The study aimed at evaluating the effect of Adansonia digitata on Cotton seed extract induced testicular damage. Method: Forty (40) Adult male Wistar rats were divided into 8 groups of five rats per group (n=5). Group1 served as the control and recieved 0.5 ml of phosphate buffer orally; Group 2 recieved 800 mg/kg b.wt A. digitata orally; Group 3 recieved 300 mg/kg b.wt Vitamin E only orally; Group 4 recieved 60 mg/kg b.wt CSE intraperitoneally; Group 5 recieved 20 mg/kg b.wt CSE intrapepritoneally; Group 6 recieved 60 mg/kg b.wt CSE intraperitoneally and 800 mg/kg b.wt A. digitata orally; Group 7 recieved 20 mg/kg b.wt CSE intraperitoneally and 800 mg/kg b.wt A. digitata orally; Group 8 recieved 60 mg/kg b.wt CSE intraperitoneally and 300 mg/kg Vit. E orally. Administration was done for a period of 21 days. The epididymis was used for semen analysis while the testes was processed for histological analysis and biochemical assay. All data were analysed by ANOVA using SPSS version 17.0 software. p<0.05 was considered significant. Results: CSE administration caused significant (p< 0.05) decrease in sperm count observed in group that was treated with CSE only. Administration of A. digitata however caused significantly increase (p< 0.05) in the sperm count, G6PDH, LDH, GPx and SOD however the MDA levels were decreased. Histology showed a increase regeneration when treated with CSE+A.D. Conclusion: The result obtained revealed the antioxidant ability of A. digitata in counter-acting the testicular damage caused by CSE administration.