Received May 07, 2020
Accepted November 20, 2020

Objective: The possible effects of ART on sex ratio at birth are extremely significant. This study aimed to determine whether the sex ratio of infants born through in vitro fertilization and embryo transfer (IVF-ET) is affected by the techniques employed in ART. Study Design: This retrospective study was conducted on 290 singleton infants born following IVF-ET from February 2014 to August 2018 at a single institution. The sex ratios of these infants were compared with respect to insemination versus intracytoplasmic sperm injection (ICSI), early-cleavage embryo versus blastocyst transfer, fresh versus frozen–thawed embryo transfer and normal sperm versus asthenospermia. Results: No significant differences in the sex ratio were observed with respect to the fertilization method, transfer time, fresh embryo or frozen–thawed embryo transfer. In addition, multiple logistic regression analysis revealed that these factors did not significantly affect the sex ratio. Conclusions: Our study indicated that the differences in the fertilization method, transfer time and sperm motility rate do not affect the sex ratio of IVF live births. However, with increasing numbers of ICSI and blastocyst transfer cycles, factors possibly affecting the sex ratio need to be further investigated.