Received May 21, 2020
Accepted November 30, 2020

Introduction: The aim of this study was to determine the correlation between semen parameters and sperm DNA fragmentation index (DFI) in 418 men who attended a fertility center. Methods: This retrospective study includes 418 male patients attended at In Vitro Gestar, Chiclayo – Perú between October 2013 and March 2020. DFI was determined between normozoospermic patients (NORMO) and patients with one altered parameter (ALTERADO). Patients were classified as oligozoospermic [Severe/Moderate oligozoospermia (OLIGO SM): <5x106 sperm/mL and Mild oligozoospermia (OLIGO M): 5 to <15x106 sperm/mL], asthenozoospermic [Severe asthenozoospermia (ASTHENO S): < 10% PM (progressive motility); Moderate asthenozoospermia (ASTHENO MO): 10 to <20% PM; Mild asthenozoospermia (ASTHENO M): 20 to <32% PM)], teratozoospermic (TERATO) and oligoasthenoteratozoospermic (OAT). DFI was determined between these groups and NORMO. The data was analyzed using SPSS 22.0 software for Windows. Results: Normozoospermic patients presented lower significant levels of DFI compared with all groups. NORMO (15.64 ± 7.65) vs [ALTERED (18.41 ± 9.43, p=0.003); OLIGO SM (26.38 ± 12.94, p<0.005); ASTHENO S (23.09 ± 11.45, p<0.01); TERATO (17.96 ± 9.23, p<0.05) and OAT (22.05 ± 12.15, p=0.001)]. We determined a significant negative correlation between DFI and progressive motility (r= -0.162 p=0.001) and with normal morphology (r= -0.100 p=0.040). Likewise the DFI shows a significant positive correlation with age (r= 0.257 p=0.000). Conclusion: Our study establishes that high DFI is accompanied by a significant impairment in all seminal parameters.