Table 1. Primer sequences used for semi-quantitative PCR analyses.
mRNA ID Primer Sequence (5’ to 3’) Product Size (bp) Annealing Temp. (°C) Cycle No.*
Nr4A1 F: GGCTTTGCTGAACTGTCT
R: GTCGGTCTGTGATGAGGA
 
271 62 25
Egr1 F: TGGCTCCTTTCCTCATTC
R: TTTGGCTGGGGTAACTCG
 
317 60 33
Gapdh** F:GGCGTGAACCACGAGAAGTATAA
R: CCCTCCACGATGCCAAAGT
 
120 60 25
*Cycle number identified to be within the linear range of amplification
**Primers from Leutenegger et al., 2000