| Table 1. Description of the used primers. | ||||
| Name | Primers |
Concentration (iM) |
Amplicon Tm (ºC) |
Amplicon Size (bP) |
|
SRY (Control) |
GAATATTCCCGCTCTCCGGA/ GCTGGTGCTCCATTCTTGAG |
125 | 86.5–86.68 | 470 |
|
AMELy (Control) |
ATCAGAGCTTAAACTGGG-AAGCTG/ CTCTGTAAAGAA- TAGTGGGTGGAT |
500 | 78.9-79.3 | 105 |
|
sY85 (AZFa) |
GCTATTCTCTCTCTGGCATC-TGTATT/ TGGCAATTTGCCTAT- GAAGT |
750 | 76.5-77.0 | 80 |
|
G34990 (AZFa) |
CATTCGGTTTTATCAGCCAG/ CAGTGACTCGAGGTTCAATG |
1500 | 76.4-76.7 | 83 |
|
sY133 (AZFb) |
ATTTCTCTGCCCTTCACCAG/ TGATGATTGCCTAAAGGGAA |
500 | 83.4-84 | 177 |
|
sY127 (AZFb) |
GGCTCACAAACGAAAAGAAA/ CTGCAGGCAGTAATAAGGGA |
300 | 81.9-82.2 | 274 |
|
sY255 (AZFc, DAZ1-4) |
GTTACAGGATTCGGCGTGAT/ CTCGTCATGTGCAGCCAC |
125 | 84.3-84.5 | 124 |
|
sY254 (AZFc, DAZ1-4) |
GGGTGTTACCAGAAGGCAAA/ GAACCGTATCTACCAAAGCAGC |
150 | 81.4 – 82.0 | 380 |