|
Table 2. Advances in sheep ovarian tissue vitrification
|
| Reference |
Tissue |
Vitrification device |
Vitrification solution |
Main outcomes |
|
Al-aghbari;
Menino, 2002.
|
Ovarian fragments (0.5cm x 0.5 cm) |
Tissue dropped on the surface of a steel cube cooled by LN2
|
35% EG, 5% polyvinylpyrrolidone, 0.4 mol/L trehalose and 20% FBS |
Maintenance of oocyte recovery rate and percentage of oocytes developing to metaphase |
|
Bordes
et al., 2005.
|
Ovarian fragments
(1 mm x 2 cm x 1 cm)
|
Cryogenic vials plunged directly into LN2
|
2.62 mol/L DMSO, 2.60 mol/L acetamide, 1.31 mol/L 1.2 PROH, and 0.0075 mol/L PEG (gradual dehydration in: 12.5%, 25%, 50% and 100% of vitrification solution) |
Pregnancies occurred after ovarian cortex autotransplantation, 4 lambs were born
|
|
Courbiere
et al., 2005.
|
Whole ovaries with
vascular pedicle
|
Samples plunged directly into LN2
|
2.75 mol/L DMSO, 2.76 mol/L formamide, and 1.97 mol/L PROH (gradual dehydration in: 12.5%, 25%, 50% and 100% of vitrification solution) |
Maintaining primordial follicle viability comparable to the
fresh tissue
|
|
Courbiere
et al., 2006.
|
Whole ovaries |
Ethyl vinyl acetate cryobag plunged into LN2
|
2.75 mol/L DMSO, 2.76 mol/L formamide, and 1.97 mol/L PROH (gradual dehydration in: 12.5%, 25%, 50% an 100% of vitrification solution) |
Maintaining the aspect of the ovarian vein identical to the
nonvitrified control
|
|
Lornage
et al., 2006.
|
Ovarian fragments
(1 mm thick and
1 cm2 surface)
|
Cryotube plunged into LN2
|
2.62 mol/L DMSO, 2.60 mol/L acetamide, 1,31 mol/L 1.2 PROH, and 0.0075 mol/L PEG |
Pregnancies occurred after autotransplantation of vitrified warmed ovarian cortex and lambs were born |
|
Baudot
et al., 2007.
|
Whole ovary with
blood vessels.
|
Ethyl vinyl acetate cryobag plunged into LN2
|
2.7 5 mol/L DMSO, 2.76 mol/L formamide and 1.97 mol/L PROH (gradual dehydration in: 12.5%, 25%, 50% and 100% of vitrification solution) |
Maintaining ovarian primordial follicle density and follicle membrane integrity similar to the fresh tissue
|
|
Courbiere
et al., 2009.
|
Whole ovary with blood vessels |
Ethyl vinyl acetate cryobag plunged into LN2
|
2.75 mol/L DMSO, 2.76 mol/L formamide, and 1.97 mol/L PROH (gradual dehydration in: 12.5%, 25%, 50% and 100% of vitrification solution) |
One sheep recovered ovarian endocrine function 6 months
after transplantation
|
|
Fathi
et al., 2011.
|
Ovarian fragments
(1 x 2 x 2 mm3)
|
Cryopin (sample adhered in a needle) plunged into LN2
|
60% HTCM, 15% EG, 15% DMSO, 0.25 mol/L SUC and 10% HSA |
Maintaining intact antral follicles using 2-step dehydration protocol
|
|
Melo
et al., 2011.
|
Ovarian fragments
(1 mm3)
|
Solid-surface plunged into LN2
|
40% EG, 0.5 mol/L SUC and 50 μg/mL AA |
Maintaining follicular viability rates similar to the fresh tissue after 5 days of tissue culture
|
|
Lunardi
et al., 2012.
|
Ovarian fragments
(3 x 3 x 1: 9 mm3)
|
Macrotube plunged into LN2
|
6 mol/L EG, 0.25 mol/L SUC and 10% FCS |
Maintaining follicular viability similar to the cultured non-
vitrified tissue after 2 days of
tissue culture
|
|
Torre
et al., 2012.
|
Whole ovaries |
Ethyl vinyl
acetate cryobags plunged into LN2
|
2.75 mol/L DMSO, 2.76 mol/L formamide and 1.97 mol/L PROH (gradual dehydration in: 12.5%, 25%, 50% and 100% of vitrification solution)
|
Significant reduction in ovarian pedicle metabolism after
vitrification
|
Note: LN