|
Table 3. Advances in women ovarian tissue vitrification
|
|
Reference
|
Tissue
|
Vitrification
device
|
Vitrification solution
|
Main outcomes
|
|
Zhang
et al., 1995.
|
Fragments of fetal
ovary (0.5 – 1 mm3)
|
Plastic cryo straws directly plunged into LN2
|
4.2 mol/L DMSO, 0.35 mol/L SUC and 15 mg/ml BSA |
Maintaining oocyte quality similar to the fresh tissue after 40 days of tissue culture |
|
Isachenko
et al., 2003.
|
Ovarian fragments
(0.8 mm x 0.8 mm x 0.8 mm)
|
Straws or grids directly plunged into LN2
|
40% EG, 0.35 mol/L SUC and 5% egg yolk extract |
Maintaining the proportion of morphologically normal follicles similar to the fresh tissue |
|
Rahimi
et al., 2003.
|
Ovarian fragments
(1 ± 0.5 mm3)
|
Straws, grids or metal filings directly plunged into LN2 or into nitrogen vapor |
40% EG, 0.35 mol/L SUC and 10% egg yolk extract or 40% EG, 18% Ficoll and 0.35 mol/L SUC |
Cooling using nitrogen vapor resulted in significantly elevated ROS levels and apoptosis after warming |
|
Rahimi
et al., 2004.
|
Ovarian fragments
(0.5 x 1.0 x 4.0 mm)
|
Straws directly plunged into LN2
|
25% Glycerol, 25% EG, 15% FCS and 1% Supercool X-100 (last solution from three steps) |
No rise in the proportion of necrotic areas after 42 days of xenotransplantation compared to fresh or slow cryopreserved tissue |
|
Gandolfi
et al., 2006.
|
Ovarian fragments
(1 mm3)
|
Straws directly plunged into LN2
|
3.58 mol/L EG, 2.82 mol/L DMSO and 20% FCS |
The addition of DMSO to the vitrification solution reduced primary follicles cryoinjuries |
|
Isachenko
et al., 2006.
|
Ovarian fragments
(1 x 1 x 5 mm)
|
Cryovials directly plunged into LN2
|
20% DMSO, 40% EG and 10% SSS (last solution from two steps) |
Morphologically normal follicles were observed when vitrified ovarian tissue was cultured for 14 days in a large volume of culture medium in combination with stirring |
|
Li
et al., 2007.
|
Ovarian fragments
(5 x 1 x 1 mm)
|
Minimum drop size directly plunged into LN2
|
2 mol/L DMSO, 2 mol/L PROH, 0.2 mol/L SUC and 12% HSA |
Maintaining estradiol and progesterone production during 14 days of tissue culture similar to the slow-frozen tissue |
|
Huang
et al., 2008.
|
Ovarian fragments
(5 x 1 x 1 mm)
|
Solid-surface
vitrification
|
20% DMSO, 20% EG, 25 mg/ml HSA (gradual dehydration in: 25%, 50%, 75% and 100% of vitrification solution) |
Maintaining the percentage of intact primordial follicles and secretion of estradiol and progesterone within 10 days of tissue culture similar to the slow-frozen tissue |
|
Isachenko
et al., 2008.
|
Ovarian fragments (about 1 mm3)
|
Droplet directly plunged into LN2
|
2.62 mol/L DMSO, 2.6 mol/L acetamide, 1.31 mol/L PROH and 0.0075 mol/L PEG (gradual dehydration in: 25%, 50%, 75% and 100% of vitrification solution) |
Maintaining normally developed follicles similar to the fresh tissue after 12 days of tissue culture |
|
Wang
et al., 2008.
|
Ovarian fragments
(~ 1–2.5 mm2)
|
Needle directly plunged into LN2
|
15% EG, 15% DMSO and 0.5 mol/L SUC |
Maintaining the ultrastructure of stromal cells better than the slow-freezing or the dropping vitrification group |
|
Kagawa
et al., 2009.
|
Ovarian fragments
(1 x 10 x 10 mm)
|
Metal strip plunged into LN2
(Cryotissue)
|
20% EG, 20% DMSO, 0.5 mol/l SUC |
Maintenance of oocyte viability similar to the fresh tissue |
|
Keros
et al., 2009.
|
Ovarian fragments
(1 x 1-2 x 5-8 mm)
|
Cryo straws
(Hand-cut straw)
|
1.4 mol/L DMSO, 1.5 mol/L EG, 1.5 PROH, 10 mg/mL HSA, and 10% PVP (last solution from three steps) |
Preservation of ovarian stroma morphology after 1 day of tissue culture better than slow freezing procedure |
|
Rahimi
et al., 2010.
|
Ovarian fragments
(~ 0.5 x 1 x 1 mm)
|
Droplet directly plunged into LN2
|
2.62 mol/L DMSO, 2.6 mol/L acetamide, 1.31 mol/L PROH and 0.0075 mol/L PEG (gradual dehydration in: 25%, 50%, 75% and 100% of solution)
|
Maintaining the revascularization of ovarian tissue similar to the frozen tissue after 30 days of xenotransplantation
|
|
Xiao
et al., 2010.
|
Ovarian fragments
(~ 2-3 mm2)
|
Needle directly plunged into LN2
|
2.15 mol/L EG, 1.69 mol/L DMSO and 0.5 mol/L SUC (last solution from two steps)
|
The use of needle immersed vitrification method enabled the use of lower cryoprotectant concentration leading to improvements in tissue cryopreservation
|
|
Zhou
et al., 2010.
|
Ovarian fragments
(1 x 1 x 1mm)
|
Direct cover
vitrification (DCV) or conventional
vitrification (CV)
|
15% EG and 15% DMSO or 20% EG and 20% DMSO
|
DCV showed a higher percentage of normal follicles and promoted less apoptotic cells compared with CV
|
|
Amorim
et al., 2011b.
|
Ovarian fragments
(1 mm3)
|
Droplet directly plunged into LN2
|
38% EG, 0.5 mol/L trehalose, 6% FBS in MEM-GlutaMAX at 10%
|
Maintaining a high percentage of normal follicles than solid-surface vitrification
|
|
Salehnia
et al., 2012.
|
Ovarian fragments
(1–1.5 mm3)
|
Cryovials or cryovials pre-cooled to 0°C put in nitrogen vapour for 30 s and then immersed LN2
|
40% EG, 30% Ficoll, 1 mol/l SUC and 1.2% BSA or increasing concentrations (2.5%, 5% and 10%) of DMSO, PROH and EG with
10% HSA
|
Maintenance of proportions of normal follicles, DNA fragmentation and ultrastructural characteristics similar to the fresh tissue after 1 day of ovarian cortex culture
|
|
Oktem
et al., 2011.
|
Ovarian fragments (0.25 cm) |
Tissue loaded into vials and immersed in LN2 |
15% PROH , 15% EG, 0.2 mol/L SUC and
10% HSA
|
Maintaining estradiol production similar to the slow-
frozen tissue after 3 days of tissue culture
|
|
Amorim
et al., 2012.
|
Ovarian fragments
(1.0 x 1.0 x 1.0 mm)
|
Solid-surface vitrification or open cryo straws |
20% DMSO, 20% EG and 25 mg/mL HSA or 10% DMSO, 26% EG, 2.5% PVP, 20 mg/mL HSA and 1 mol/L SUC |
Percentage of follicles with DNA damage lower than in the slow-frozen tissue after 7 days of xenotransplantation
|
|
Kawamura
et al., 2013.
|
|
|
|
Pregnancy and birth |
Note: LN