Table 3

Table 3. Mean percent value (± standard error) of cells in Class I, II, III and IV, for 3,3’-diaminobenzida staining to assess mitochondrial activity in spermatozoa of fresh samples and different groups tested.
	PRE-FREEZING (COOL)	Post-Freeze
	PRE-FREEZING (COOL)	GROUP 1 (CONTROL)	GROUP 2 (DRY ICE)	GROUP 3 (DRY ICE + NITROGEN)	GROUP 4 (TRANSPORTED)	GROUP 5 (TRANSPORTED + NITROGEN)
Class I (%)	25.8 ± 1.6^a	25.3 ± 1.8^a	23.7 ± 1.4^a	24.4 ± 1.5^a	24.3 ± 1.7^a	24.5 ± 1.4^a
Class II (%)	50 ± 1.5^a	49.2 ± 1.5^a	49.4 ± 1.2^a	48.8 ± 1.3^a	48.2 ± 1.4^a	48.3 ± 1.3^a
Class III (%)	15.4 ± 1.1^a	14.8 ± 1.2^a	15.9 ± 1.1^a	15.6 ± 1.0^a	15.5 ± 1.1^a	14.8 ± 1.1^a
Class IV (%)	8.8 ± 0.8^a	10.6 ± 1.0^a	10.9 ± 0.8^a	11.1 ± 0.7^a	11.9 ± 0.9^a	12.3 ± 0.7^b
*Different letters in the same row differ. P <0.05.