| Table 2. Embryo quality according to the ASEBIR guidelines on D-2 and D-3 for embryos cultured in single medium or sequential media | |||
| Class | No. embryos Single medium (%) | No. embryos Sequential media (%) | P |
| D+2 | n=486 | n=472 | |
| A | 190 (39) | 107 (23) | <0.001 |
| B | 133 (27) | 118 (25) | 0.018 |
| C | 154 (32) | 215 (45) | NS |
| D | 9 (2) | 32 (7) | 0.012 |
| D+3 | n=141 | n=145 | |
| A | 40 (28) | 19 (13) | 0.048 |
| B | 40 (28) | 49 (34) | NS |
| C | 47 (34) | 59 (41) | NS |
| D | 14 (10) | 18 (12) | NS |
| No: number. NS: not significant. | |||