Figure 4. Flow cytometry of Annexin / PI staining in the experimental & control groups. Lower left quadrant: live cells; Lower right quadrant: initial apoptosis; Upper right quadrant: late apoptosis; Upper left quadrant: necrotic cells (A and B). The relative level of mRNA expression of Bax and Bcl-2 genes and Bax/Bcl-2 ratio (C and D). The values are expressed as mean±SD. *p<0.05, **p<0.01, ^#p<0.05, ^##p<0.01, ^###p<0.001, ^$p<0.05, ^$$p<0.01; *, ^# and the ^$ symbols respectively indicate comparison to the control, 3-MA and TAU groups.