Figure 1. Scheme of the methodology used in the study. In vitro oocyte maturation was performed in microdroplets of 30μL of Sydney IVF Fertilization Medium at 37ºC, 5% CO2 and high humidity. VI-2: vitrification solution, DV-1, and DV-2: devitrification solutions containing 1M and 0.5M sucrose respectively (Ingámed®) were used to induce a temporary hyperosmotic shock. N: number of immature oocytes in each group.