Table 1

Gene	Chromosome	Polymorphism^a	Molecular biology techniques	Primer^a
Table 1. Studied gene polymorphisms
GDF9^b	5	c.398-39 C>G (intron)	Direct sequencing
		p.Thr149Thr (c.447C>T)		Forward: 5' TTGACTTGACTGCCTGTTGTG 3'
		p.Glu186Glu (c.546G>A)		Reverse: 5’ AGCCTGAGCACTTGTGTCATT 3’
		p.Val216Met (c.646G>A)
		p.Val216Met (c.646G>A)
FSHR^c	2	p.Asn680Ser	RFLP - restriction enzyme BsrI	Forward: 5' TTGTGGTCATCTGTGGCTGC 3'
FSHR^c	2	p.Asn680Ser	RFLP - restriction enzyme BsrI	Reverse: 5' AAAGGCAAGGACTGAATTATCATT 3'
LHβ^d	19	p.Trp8Arg	Direct sequencing	Forward: 5’ GAAGCAGTGTCCTTGTCCCA 3’
LHβ^d	19	p.Ile15Thr	Direct sequencing	Reverse: 5’ GAAGAGGAGGCCTGAGAGTT 3’ Reverse: 5’ GGAGGGAAGGTGGCATAGAG 3’
LHCGR^e	2	18insLQ	Direct sequencing	Forward: 5’ CACTCAGAGGCCGTCCAAG 3’
AMH^f	19	p.Ile49Ser (c.146T>G)	Taqman	Assay ID: C_25599842_10
AMHR2^g	12	c.-482A>G	Taqman	Assay ID: C_1673084_10
BMP15^h	X	c.-9C>G	Taqman	Assay ID: C_27504454_10

^a C: cytosine, T: thymine, A: adenine, G: guanine
^bHuman growth differentiation factor 9
^cFollicle-stimulating hormone receptor
^dhBone morphogenetic protein 15