Table 2. Media usage through various species and follicular stages.
Culture Medium Species Follicle Stage References
Whitten’s medium Murine PGOC Eppig, 1980
Bicarbonate buffered M199 Murine Small follicles Torrance et al., 1989
Waymouth’s medium Murine PGOC Eppig, 1991
O’Brien et al., 2003
Human Immature Laronda et al., 2014
Ovine Primordial and primary (40–60µm) Muruvi et al., 2005
Porcine Preantral (200–300µm) Hirao et al., 1994
Way/IBMX/lTS/BSA medium Bovine Preantral (60 to 179µm) Wandji et al., 1996
  Murine Preantral and COC Vanderhyden et al., 1992
DMEM Human Class 1 (90µm)
Class 2 (<90µm)
Roy & Treacy, 1993
Preantral (90–240µm) Yuan & Guidice, 1999
Marsupial Primordial and primary (63.6–215.5µm) Nation & Selwood, 2009
αMEM Baboon Preantral (270–300µm) Xu et al., 2011b
Bovine Preantral (190.0±6.6µm) Araújo et al., 2014
Secondary (≥150µm) Rossetto et al., 2013a
Caprine Secondary (≥150µm) Rossetto et al., 2013a
Secondary (≥150µm) Rossetto et al., 2013a
Preantral (≥200µm) Magalhães et al., 2011
Preantral (150–250µm) Silva et al., 2015
Preantral (~250µm)
early antral (~350µm)
Ferreira et al., 2018
Canine Pre- and early antral (100–500µm) Songsasen et al., 2011
Feline Secondary (208±7.9µm)
Early antral (329.8±5.4µm)
Songsasen et al., 2017
Secondary (100-200µm) Thongkittidilok et al., 2018
Human Pre- and early antral (≥120µm) Abir et al., 1997
Secondary (170–178µm) Xu et al., 2009b
Secondary (176.46±7.20µm) Laronda et al., 2014
Murine COC Pangas et al., 2003
Preantral (150–200µm) Adam et al., 2004
Preantral (180–240µm) Wycherley et al., 2004
Antral (≥200 µm) Miller et al., 2005
Two-layered (100–130µm)
Multi-layered (150–180µm)
Kreeger et al., 2005; 2006
Two-layered (100–130µm) Xu et al., 2006b
Shikanov et al., 2009
Desai et al., 2012
Multi-layered secondary (150–180µm) Xu et al., 2006a
Preantral (~60–69µm) Oktem & Oktay, 2007
Secondary (111–137µm) Jin et al., 2010
Antral (360.94±16.1µm) Craig et al., 2010
Immature secondary (140–150µm) Shikanov et al., 2011
Secondary (~90, 100–105, or 120µm) Tingen et al., 2011
    Early preantral (100–130µm) Adriaens et al., 2004
Antral (200–350µm) Craig et al., 2013
Antral (250–400µm) Peretz & Flaws, 2013
Peretz et al., 2013
Hannon et al., 2015
Zhou & Flaws, 2017
Patel et al., 2016
Preantral (85–115µm) Hornick et al., 2013
Primary (60–80 µm)
two-layered (90–100µm)
Antral (225–400µm)
Tagler et al., 2014
Mahalingam et al., 2016a; 2016b
Secondary (180–210µm) Skory et al., 2015
Preantral (150-160µm) Heise et al., 2005
Preantral (140-170µm) Heise et al., 2009
Early secondary (100-130µm) Mainigi et al., 2011
Ovine Preantral (small 130±10µm)
Preantral (medium 185±14µm)
Preantral large (250±10µm)
Cecconi et al., 1999
Rhesus Secondary (100–300µm) Xu et al., 2009a
Secondary (125–225µm) Xu et al., 2011a
Xu et al., 2010
Rodrigues et al., 2015
Primary (80–120µm)
secondary (125–225µm)
Xu et al., 2013
Small antral (≥0.5mm) Peluffo et al., 2013
Secondary (125–250µm) Ting et al., 2015
Baba et al., 2017
Secondary (130–220µm) Xu et al., 2015b
Secondary (140–225µm) Xu et al., 2018
αMEM + F12 Human Multi-layered (165.8±32.3µm) Xiao et al., 2015
αMEM + Glutamax Human Preantral (190±30µm) Aziz et al., 2017
Secondary (74–260µm) Skory et al., 2015
Small preantral (42.98±9.06µm) Amorim et al., 2009
αMEM + TCM199 Bovine Preantral (≥190µm) Araújo et al., 2015
αMEM + Earle’s balanced salts Murine COC and PGOC Buccione et al., 1990
Diaz et al., 2007
Sugiura et al., 2010
TCM199B Bovine Preantral (40–70µm) Schotanus et al., 1997
Preantral (145–170µm) Itoh et al., 2002
Secondary (268.6±4.5µm) Antonino et al., 2019
Ovine Preantral (250–400µm) Arunakumari et al., 2010
Earle’s Balanced Salts Human Primary (47.0±8.2µm) Abir et al., 1999
McCoy’s 5a Bovine Preantral (166±2.15µm) Gutierrez et al., 2000
Human Preantral (66 to 132µm) Telfer et al., 2008
Secondary (≥100µm) McLaughlin et al., 2014
Primordial/primary follicle (≤60µm)
primary/early secondary follicle (>60-120µm)
Secondary (>120–250µm)
Yin et al., 2016
Secondary (100–150µm) McLaughlin et al., 2018
Ovine Preantral (161±2µm) Thomas et al., 2003
NUSC-23 Media Porcine Preantral (296±9µm) Wu et al., 2001
TALP Rhesus COC Peluffo et al., 2010; 2012