Figure 3. Immunohistochemistry of rat testis for BCL-2, p53 and BAX expression. Saline treated control group animals showing negative stain for BCL-2 and p53 in testis (A & B, 200X.) and BAX positive basal cells and interstitial cells (C, 200X). Cleistanthus collinus 200 mg/kg treated animals showing BCL-2 positivity in sperm heads of spermatid and negative in other spermatogenic cells (D), weak p53 positivity (E) and BAX positivity in sperm cells (F). IHC 200X Cleistanthus collinus 400 mg/kg group showing BCL-2 positivity in mature sperm and negative in immature sperm cells (G), p53 focal nuclear positivity in spermatocytes (H) and BAX positivity in the spermatozoa(I). IHC 200X.