| Table 1. Studies that used spent culture medium to validate NiPGT. | ||||||
| Samples | Analyze | |||||
|---|---|---|---|---|---|---|
| Development Day | Number of samples | culture medium | Agreement | Amplification Kits | Cytogenetics Method | |
| Spent culture medium
Shamonki et al . (2016) Xu et al . (2016) Feichtinger et al . (2017) Liu et al. (2017) Ho et al . (2018) Huang et al . (2019) Vera-Rodriguez et al . (2018) Yin et al . (2021) Rubio et al . (2019) Yeung et al. (2019) |
D3-5/6 D3-5 D1-5 D1-5 D3-5 D5/6 D3-5 D5 D4-5/6/7 D3-5/6 |
57 42 22 88 41 52 56 75 115 168 |
two steps two steps single step single step single step two steps two steps two steps two steps two steps |
3.5% 85.7% 27% 64.5% D3-56.3%/D5-65% 93.8% 30.4% 27.2% D5-78.7%/D6/7- 84% 62.1% |
Repli-G MALBAC SurePlex MALBAC Picoplex MALBAC SurePlex ChromInst ThermoFisher SurePlex |
CGH NGS CGH NGS NGS NGS CGH NGS NGS NGS |
| Spent culture medium andblastocoel fluid
Kuznyetsov et al . (2018) Jiao et al . (2019) |
D5-6/7 D5/6 |
47 62 |
two steps two steps |
87.5% 76.1% |
SurePlex MALBAC |
NGS NGS |
Main studies reporting the use of spent culture medium for preimplantation genetic testing. D1-7, day of embryonic development; NGS, next-generation sequencing; CGH, comparative genomic hybridization.