Figure 4. miR-181d-5p expression in murine ovarian tissue. (a) Total RNA was extracted from the ovaries removed from ovariectomized animals (31 days-old) and from the ovaries removed from female mice from CTR group at the end of the experiment (50 days-old). RNU6B was used as an endogenous control. (b) Small RNA was extracted from blood collected from the ovariectomized (OVA) and the control group (CTR) animals. Spiked-in cel-miR-39 was used for the normalization of the serum miRNA quantification.