Table 1. Microbiota assessment depending on seminal quality. Normospermia (normal semen parameters), oligoasternospermia (low concentration and low sperm motility), asternosperm (low sperm motility), azoospermia (absence of sperm in the ejaculate), oligospermia (low concentration of sperm) and leukocytospermia (increase in the number of leukocytes in semen.
Author Year Number of individuals Classification according to seminal quality Microorganisms present in each group Methodology
Hou et al. 2013 77 Healthy sperm donors Streptococcus; Corynebacterium;
Finegoldia; Veillonella;
Lactobacillus; Prevotella;
Staphylococcus; Anaerococcus;
Peptoniphilus
16 S rRNA gene
sequencing and
Gram stain
Asthenospermia Prevotella; Peptoniphilus;
Lactobacilus; Clostridiales
Oligoasthenospermia Corynebacterium;
Staphylococcus; Finegoldia;
Anaerococcus
Oligoasthenozoospermia
and severe azoospermia
Ralstonia; Prevotella;
Aerococcus; Gemella
Baud et al. 2019 94 Normal spermogram
parameters
Staphylococcus; Lactobacillus 16 S rRNA gene
sequencing
One or more abnormal
parameters
Prevotella
Yang et al. 2020 159 Control men Acinetobacter; Pelomonas;
Streptococcus; Lactobacillus
16 S rRNA gene
sequencing
Oligoasthenospermia Ralstonia; Bacteriodes;
Prevotella; Lactobacillus
Asthenospermia Sneathia; Ralstonia;
Ureaplasma; Aerococcus;
Anaerococcus; Corynebacterium
Azoospemia -
Oligospermia -
Yao et al. 2022 87 control men Lactobacillus ↑; Streptococcus 16 S rRNA gene
sequencing
Asthenospermia
Leukocytospermia Streptococcus
Asthenospermia and WBC
count >1x106ml
Lactobacillus
Lundy
et al.
2021 37 Control men Collinsella ↑; Pseudomonas 16 S rRNA gene
sequencing
and shotgun sequencing
Men with idiopathic
infertility
Aerococcus; Gardnerella;
Prevotella; Pseudomonas;
Collinsella
Puerta Suárez & Cardona Maya 2021 10 Group control Rhizobiaceae; Burkholderia;
Achromobacter; Delftia;
Campylobacter; Ezakiella;
Anaerococcus; Prevotella;
Haemophilus
16 S rRNA gene sequencing
Prostatitis group
Okwelog
et al.
2021 36 Normospermic men Lactobacillus; Gardnerella;
Veillonella; Corynebacterium;
Escherichia coli; Hemophilus;
Prevotella
16 S rRNA gene sequencing
Men with oligospermia Prevotella; Escherichia coli;
Lactobacillus; Gardnerella