Table 1. Chromatin configuration and extrusion rate after in vitro maturation of bovine oocytes in conventional (2D) or collagen membrane associated (3D) culture systems.
Treatments Chromatin configuration (%) Extruded (%)
GV MI MII
CC-2D 32.6 (47/144)A 7.6 (11/144)AB 59.0 (85/144)B 0.7 (1/144)A
CC-3D 27.0 (34/126)A 4.0 (5/126)A 60.3 (76/126)B 8.7 (11/126)B
SC-2D 43.8 (64/146)B 10.9 (16/146)B 44.5 (65/146)A 0.6 (1/146)A
SC-3D 46.3 (64/138)B 5.7 (8/138)AB 47.1 (65/138)A 0.7 (1/138)A

A,B Different letters in the column indicate statistical significance (p<0.05).
Oocytes with cumulus (CC) or without cumulus (SC) submitted to conventional MIV (2D) or with collagen matrix (3D).
GV: germinal vesicle; MI: metaphase I; MII: metaphase II.