Table 2. Stages of nuclear maturation, and percentages of normal MII oocytes matured in vitro in medium without follicular fluid (No-FF), with addition of 1% FF from infertile patients without endometriosis (FFC), with early endometriosis (FFEI/II), with advanced endometriosis without (FFEIII/IV) or with endometrioma (FFEndometrioma), supplemented with 0.6 mg/mL L-carnitine and 1 nM omega-3 (LC+n3) visualized by confocal microscopy.
Fixed oocytes after IVM n Viewed by confocal N MI
n (%)
TI
n (%)
PA
n (%)
MII
Total MII
n (%)
Analyzable
n (%)
Normal
n (%)
No-FF 167 149 9(6.0%)a 2 (1.3%) 1 (0.7%) 137 (91.9%)a 94 (68.6%) 82 (87.2%)a
FFC 163 148 9 (6.1%)a 5 (3.4%) 2 (1.4%) 132 (89.2%)ab 78 (59.1%) 68 (87.2%)a
FFC+LC+n3 179 163 19 (11.7%)ab 3 (1.8%) 3 (1.8%) 138 (89.2%)ab 80 (58.0%) 66 (82.5%)ab
FFEI/II 178 158 20 (12.7%)ab 1 (0.6%) 2 (1.3%) 135 (85.4%)ab 82 (60.7%) 51 (62.2%)c
FFEI/II
+LC+n3
186 163 16 (9.8%)ab 4 (2.5%) 4 (2.5%) 139 (85.3%)ab 84 (60.4%) 71 (84.5%)ad
FFEIII/IV 185 166 28 (16.9%)bc 2 (1.2%) 2 (1.2%) 134 (80.7%)b 84 (62.7%) 59 (70.2%)bc
FFEIII/IV
+LC+n3
163 142 11 (7.7%)a 1 (0.7%) 1 (0.7%) 129 (90.8%)a 82 (63.6%) 69 (84.1%)ad
FFEndometrioma 163 150 37 (24.7%)c 7 (4.7%) 2 (1.3%) 104 (69.3%)c 66 (63.5%) 48 (72.7%)bcd
FFEndometrioma
+LC+n3
177 162 18 (11.1%)ab 0 (0%) 0 (0%) 4 (2.5%) 140 (86.4%)ab 85 (60.7%) 64 (75.3%)abc

Note: IVM: in vitro maturation; MI=metaphase I; TI=telophase I; MII=metaphase II; PA=parthenogenetic activation. Analyzable: oocytes with spindles fixed in lateral or sagittal view. Data are results of 8 replicates using follicular fluid from control patients, with mild endometriosis, with severe endometriosis without endometrioma and with endometrioma. Different letters in the same column indicate statistical difference, p <0.05, using chi-square test.