| Table 2. Stages of nuclear maturation, and percentages of normal MII oocytes matured in vitro in medium without follicular fluid (No-FF), with addition of 1% FF from infertile patients without endometriosis (FFC), with early endometriosis (FFEI/II), with advanced endometriosis without (FFEIII/IV) or with endometrioma (FFEndometrioma), supplemented with 0.6 mg/mL L-carnitine and 1 nM omega-3 (LC+n3) visualized by confocal microscopy. | ||||||||
| Fixed oocytes after IVM n | Viewed by confocal N | MI n (%) |
TI n (%) |
PA n (%) |
MII | |||
|---|---|---|---|---|---|---|---|---|
| Total MII n (%) |
Analyzable n (%) |
Normal n (%) |
||||||
| No-FF | 167 | 149 | 9(6.0%)a | 2 (1.3%) | 1 (0.7%) | 137 (91.9%)a | 94 (68.6%) | 82 (87.2%)a |
| FFC | 163 | 148 | 9 (6.1%)a | 5 (3.4%) | 2 (1.4%) | 132 (89.2%)ab | 78 (59.1%) | 68 (87.2%)a |
| FFC+LC+n3 | 179 | 163 | 19 (11.7%)ab | 3 (1.8%) | 3 (1.8%) | 138 (89.2%)ab | 80 (58.0%) | 66 (82.5%)ab |
| FFEI/II | 178 | 158 | 20 (12.7%)ab | 1 (0.6%) | 2 (1.3%) | 135 (85.4%)ab | 82 (60.7%) | 51 (62.2%)c |
| FFEI/II +LC+n3 |
186 | 163 | 16 (9.8%)ab | 4 (2.5%) | 4 (2.5%) | 139 (85.3%)ab | 84 (60.4%) | 71 (84.5%)ad |
| FFEIII/IV | 185 | 166 | 28 (16.9%)bc | 2 (1.2%) | 2 (1.2%) | 134 (80.7%)b | 84 (62.7%) | 59 (70.2%)bc |
| FFEIII/IV +LC+n3 |
163 | 142 | 11 (7.7%)a | 1 (0.7%) | 1 (0.7%) | 129 (90.8%)a | 82 (63.6%) | 69 (84.1%)ad |
| FFEndometrioma | 163 | 150 | 37 (24.7%)c | 7 (4.7%) | 2 (1.3%) | 104 (69.3%)c | 66 (63.5%) | 48 (72.7%)bcd |
| FFEndometrioma +LC+n3 |
177 | 162 | 18 (11.1%)ab | 0 (0%) | 0 (0%) 4 (2.5%) | 140 (86.4%)ab | 85 (60.7%) | 64 (75.3%)abc |
Note: IVM: in vitro maturation; MI=metaphase I; TI=telophase I; MII=metaphase II; PA=parthenogenetic activation. Analyzable: oocytes with spindles fixed in lateral or sagittal view. Data are results of 8 replicates using follicular fluid from control patients, with mild endometriosis, with severe endometriosis without endometrioma and with endometrioma. Different letters in the same column indicate statistical difference, p <0.05, using chi-square test.