| Table 1. Stages of nuclear maturation, and percentages of normal MII oocytes matured in vitro in medium without follicular fluid (No-FF), with addition of 1% FF from infertile patients without endometriosis (FFC), with early endometriosis (FFEI/II) and with advanced endometriosis (FFEIII/IV) visualized by confocal microscopy. | |||||||
| Oocytes viewed by confocal |
MI n (%) |
TI n (%) |
PA n (%) |
MII | |||
|---|---|---|---|---|---|---|---|
| Total MII n (%) |
Analyzable n (%) |
Normal n (%) |
|||||
| No-FF | 101 | 20 (19.8%) | 1 (1.0%) | 4 (4.0%) | 76 (75.2%) | 46 (60.5%) | 37 (80.4%)a |
| FFC | 124 | 23 (18.7%) | 1 (0.8%) | 4 (3.3%) | 96 (78.0%) | 56 (58.3%) | 45 (80.4%)a |
| FFEI/II | 99 | 18 (18.2%) | 3 (3.0%) | 5 (5.1%) | 73 (73.7%) | 40 (54.8%) | 23 (57.5%)b |
| FFEIII/IV | 158 | 41 (25.9%) | 2 (1.3%) | 3 (1.9%) | 112 (70.9%) | 62 (55.4%) | 41 (66.1%)ab |
Note: MI=metaphase I; TI=telophase I; MII=metaphase II; PA=parthenogenetic activation. Analyzable: oocytes with spindles fixed in lateral or sagittal view. Data are results of 4 replicates using follicular fluid from control patients, with mild endometriosis and with severe endometriosis. Different letters in the same column indicate statistical difference, p<0.05, using chi-square test.