Table 2. Cell numbers in blastocysts derived from intact and half-destroyed 4-cell mouse embryos, cultured in medium with or without 20 µg/ml crocin.
Blastocysts from Intact
4-Cell Embryos
p-value

Blastocysts from
Half-Destroyed 4-Cell
Embryos

p-value
Control
(n=47)
Crocin
(n=45)
Control
(n=36)
Crocin
(n=51)
ICM 27.9±9.7 26.7±1.4 0.887 13.7±2.9 14.5±3.9 0.285
TE 67.7±21.8 72.9±17.7 0.525 46.0±13.3 45.2±12.3 0.764
Total cells 95.6±28.2 99.6±23.5 0.831 59.7±14.8 59.7±12.2 0.990
ICM:TE Ratio 0.4±0.2 0.4±0.1 0.162 0.3±0.1 0.4±0.1 0.272

Values are presented as mean ± SD.
Non-paired t-tests
ICM = Inner cell mass, TE = trophectoderm
Some blastocysts were excluded due to accidental loss or poor staining.