Table 1. The comparison of blastocyst rate, hatching rate, and total cell number in study groups.
Groups Number of Morulae Blastocyst Rate (%) Hatching Rate (%) TCN
VS-/PW- 9 93.75 ab 28.57 abcd 67.00
VSe10/PWe10 8 92.86 ab 20.00 abce 61.00
VSe30/PWe30 7 84.52 ab 20.00 abce 58.00
VS+10/PW- 9 88.75 ab 50.00 acde 63.00
VS-/PW+10 9 95.00 ab 40.00 acde 66.00
VS+10/PW+10 8 95.00 ab 66.67bcde 63.67
VS+30/PW- 7 78.89 ab 28.57 abcd 68.5
VS-/PW+30 8 28.89 0.01 abde 54.5
VS+30/PW+30 8 23.21 16.67 abce 52.00

All data is drawn from two replicates. Data are reported as Mean. abcde: significant difference between groups (p≤0.05). In the comparison of blastocyst rate: a vs VS-/PW+30, b vs VS+30/PW+30. In the comparison of hatching rate: a vs VS+10/PW+10, b vs VS-/PW+10 and VS+10/PW-, c vs VS-/PW+30, d vs VS+30/PW+30, VSe10/PWe10, and VSe30/PWe30 and e vs VS-/PW- and VS+30/PW-. In the comparison of TCN, there was no significant difference between the studied groups. VS: Vitrification Solution, PW: Post Warming, e10: presence of 10 µL/ml ethanol, e30: presence of 30 µL/ml ethanol, +10: presence of 10 µM CoQ10, +30: presence of 30 µM CoQ10, -: absence of CoQ10.