| Table 1. The comparison of blastocyst rate, hatching rate, and total cell number in study groups. | ||||
| Groups | Number of Morulae | Blastocyst Rate (%) | Hatching Rate (%) | TCN |
|---|---|---|---|---|
| VS-/PW- | 9 | 93.75 ab | 28.57 abcd | 67.00 |
| VSe10/PWe10 | 8 | 92.86 ab | 20.00 abce | 61.00 |
| VSe30/PWe30 | 7 | 84.52 ab | 20.00 abce | 58.00 |
| VS+10/PW- | 9 | 88.75 ab | 50.00 acde | 63.00 |
| VS-/PW+10 | 9 | 95.00 ab | 40.00 acde | 66.00 |
| VS+10/PW+10 | 8 | 95.00 ab | 66.67bcde | 63.67 |
| VS+30/PW- | 7 | 78.89 ab | 28.57 abcd | 68.5 |
| VS-/PW+30 | 8 | 28.89 | 0.01 abde | 54.5 |
| VS+30/PW+30 | 8 | 23.21 | 16.67 abce | 52.00 |
All data is drawn from two replicates. Data are reported as Mean. abcde: significant difference between groups (p≤0.05). In the comparison of blastocyst rate: a vs VS-/PW+30, b vs VS+30/PW+30. In the comparison of hatching rate: a vs VS+10/PW+10, b vs VS-/PW+10 and VS+10/PW-, c vs VS-/PW+30, d vs VS+30/PW+30, VSe10/PWe10, and VSe30/PWe30 and e vs VS-/PW- and VS+30/PW-. In the comparison of TCN, there was no significant difference between the studied groups. VS: Vitrification Solution, PW: Post Warming, e10: presence of 10 µL/ml ethanol, e30: presence of 30 µL/ml ethanol, +10: presence of 10 µM CoQ10, +30: presence of 30 µM CoQ10, -: absence of CoQ10.