Figure 3. Histological characteristics of fresh and frozen-thawed testis grafts in the epididymal fat region. Fresh and frozen-thawed mouse neonatal testis tissue stained with hematoxylin and eosin (H&E) before and after grafting. A: Fresh neonate testis tissue (before graft). B: Frozen-thawed neonate testis tissue (before graft). C: Adult testis tissue. D-F: Fresh grafted testis tissue (8 weeks after graft). G-I: Frozenthawed grafted testis tissues (n8 weeks after graft). The insert depicts the area with elongated spermatids.